May 10, 2023 · characteristics for use as a new topical antimicrobial agent to treat mupirocin-resistant MRSA SSTIs and would be a promising over-the-counter (OTC) medication in place of topical mupirocin or fusidate given its low likelihood to develop cross-resistance from or to conventional antibiotics. 5,6 It is not re- lated to any other known class of anti-infective agents in terms of its chemical structure (Fig. 07 ± 0. The different bonds between the nucleoside and amino acid moieties are shown in pink. 1 Mechanism of Action Mupirocin inhibits bacterial protein synthesis by reversibly and specifically binding to bacterial isoleucyltransfer RNA (tRNA) synthetase. 09 at 1 h, 5. Jun 1, 1989 · Mupirocin (the active component of Bactroban ointment) is the approved name for pseud0monic acid A, a naturally occurring metabolite derived from submerged cul- tures of Pseudomonas fluorescens. Penicillin‐binding proteins and the mechanism of action of beta‐lactam antibiotics. 3% systemic absorption. Pharmacology. Of the organisms detected before Apr 21, 2009 · Preparation of Mupirocin-Loaded Emulgel. mentagrophytes ringworm model in guinea pigs. 1. Absorption. 2 The molecule itself is a cationic bis May 10, 2023 · Since the mechanism of action by Fe(8-hq) 3 against SA bacteria is completely nonoverlapping with the molecular targets of other conventional antibiotics, Fe(8-hq) 3 may therefore have potential as an alternative to both mupirocin and fusidate for treating SSTIs by MRSA. . Nasal decolonization of MRSA: Apply to nares q 12h for 5 days. Mupirocin selectively inhibits the bacterial isoleucyl-tRNA synthetase (IleRS), leading to the accumulation of uncharged isoleucyl-tRNA and eventually the synthesis of (p)ppGpp. Mometasone furoate also has a lower affinity to mineralocorticoid receptors than natural corticosteroids, making it more selective in its action 2. Feb 17, 2020 · Mupirocin is a topical antibiotic that binds to bacterial isoleucyl transfer-RNA synthetase and inhibits protein synthesis. aureus Jan 8, 2024 · Mechanism of Action. When administered topically, mupirocin was efficacious in a T. The biosynthetic cluster encodes 6 type I polyketide synthase multifunctional proteins and 29 single function proteins. Mupirocin ointment has a unique chemical structure and antibacterial activity. mupirocin (Bactroban), A woman who is taking isotretinoin calls the office to say that she thinks she may be pregnant. Mechanism of action. 2A) . Pharmacological Classification: Antibacterial (Antibiotic for Topical Use). 2014 ). Mar 29, 2019 · Mechanism of action, resistance, properties Pseudomonic acid A represents 90–95% from the mixture and is the main compound of Mupirocin (Bojarska et al. Mupirocin, formerly termed pseudomonic acid A, is a topical antibiotic under investigation for the treatment of impetigo and other superficial primary and secondary skin infections. mupirocin is pseudomonic acid & is a natural product produced by Pseudomonas fluorescens. Mupirocin Doses. 10. Results and Discussion 2. 35 mupirocin, available reports have not observed a link between PVP -I and the induction 106 Mechanism of action and a ntimicrobial spectrum . It has a broad antibacterial activity on Gram positive and some Gram negative bacteria, and a limited systemic adsorption: the half-life is 15 min, being converted into monic acid Fusidic acid is a bacteriostatic antibiotic and helps prevent bacterial growth while the immune system clears the infection. Davis PT Collection is a subscription-based resource from McGraw Hill that features trusted content from the best minds in PT. Generic Name Chlorhexidine DrugBank Accession Number DB00878 Background. Mupirocin inhibits bacterial protein synthesis by reversibly and specifically binding to bacterial isoleucyl transfer-RNA synthetase. It has a unique structure, mechanism of action, and water-miscible formulation. Excretion: urine <3%. When it is applied to an infected area three times daily, clinical improvement should be seen in 85% to 100% of patients within 3 to 5 days. aureus nasal carriage is a risk factor for subsequent infections, especially amongst surgical and dialysis patients [2, 3], and carriage of methicillin-resistant S. However, the MupA gene may co-transfer with other antibacterial resistance genes. This antibiotic has a unique chemical structure and mechanism of action. Because of its unique structure and mechanism of action, mupirocin has little, if any, potential for cross-resistance with other antibiotics. This medicine contains mupirocin, a topical antibacterial that helps heal minor cuts and wounds in the skin and is used to treat or prevent skin infections caused by Staphylococcus aureus and Streptococcus pyogenes . Inhibits bacterial protein synthesis by binding with the bacterial transfer-RNA. Davis PT Collection. It is mainly efficacious against Gram-positive aerobes such as staphylococci. Mupirocin inhibits bacterial protein synthesis by reversibly and specifically binding to bacterial isoleucyl-transfer RNA (tRNA) synthetase. Mupirocin is available as a 2% topical ointment. Here, we provide the largest and most comprehensive data set to use RNA sequencing technology for the purpose of characterizing the bacterial response to Mechanism of action. 13 Triamcinolone can be used as a one time adjunct treatment of osteoarthritic knee pain, 16 or first line as a topical treatment of corticosteroid responsive The chemistry, mechanism of action, antimicrobial activity, pharmacokinetics, clinical efficacy, adverse effects, dosage, and administration of mupirocin are reviewed. Disturbances in sodium concentrations in the extracellular fluid are associated with disorders of water balance. Mupirocin was significantly more effective in the treatment of acute primary skin infections and in the treatment of a subgroup of patients with impetigo (P < 0·01). As vitamin K is a cofactor for the carboxylation of glutamate residues on the N-terminal regions of vitamin K-dependent clotting factors, this limits the gamma-carboxylation and subsequent activation of the Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. bacitracin B. Mar 27, 2023 · Mechanism of Action. Do not apply mupirocin ointment concurrently with any other lotions, creams, or ointments [see Clinical Pharmacology (12. The nurse anticipates use of which medication to treat the MRSA? A. Jul 1, 1989 · Mupirocin is a new, topical antibiotic effective for the treatment of impetigo. The most frequent side effects are burning, stinging, pain, and itching at the area of In the present study, we analyzed the response of S. 05) ( 47 ). The obtained emulsion was added to sepigel with gentle stirring to get the emulgel base. Mupirocin 2% Cream; Mupirocin 2% Ointment. Clinically, the frequency of staphylococcal resistance to it and to mupirocin has remained low. (A) Chemical structures of the 3′ end of an aminoacylated tyrosyl-tRNA (left) and puromycin (right). 1979. DB00257. Annu Rev Biochem 52:825–869. Mupirocin, also known as pseudomonic acid A, is a short-chain fatty acid and the primary fermentation metabolite of P fluorescens. Nov 23, 2015 · Herein, we set out to improve the performance of mupirocin-based ointments. It works by inhibiting bacterial protein synthesis, which causes the bacteria to die. The SNP of 5% PVP-I was also significantly more effective than mupirocin against low-level and high-level mupirocin-resistant MRSA isolates in this porcine model (P 0. Mupirocin is an investigational topical antibiotic used for treatment and prophylaxis of bacterial skin infections. (The figure was adopted and reproduced from Thomas et al. 3)]. Such H1 receptors are located on respiratory smooth muscles, vascular endothelial cells, the gastrointestinal tract (GIT), cardiac tissue, immune cells, the uterus, and the central nervous system (CNS) neurons 9 , 10 Read this chapter of Davis's Drug Guide for Rehabilitation Professionals online now, exclusively on F. polymyxin B D. Distribution. Chlorhexidine is a broad-spectrum antimicrobial biguanide used as a topical antiseptic and in dental practice for the treatment of inflammatory dental conditions caused by microorganisms. Mechanism of Action: Inhibition of the DNA replication enzymes DNA gyrase A and topoisomerase IV. Study with Quizlet and memorize flashcards containing terms like The nurse is assessing a patient who has been diagnosed with MRSA on the right arm. Dec 31, 2015 · Mechanism of action Diphenhydramine predominantly works via the antagonism of H1 (Histamine 1) receptors 9 , 10 , 11 , 17 , 5 . Mupirocin Nasal Ointment (Parafin based) (1g) Mupirocin Ointment (15, 30g) Muprocin Ointment (Polyethylene glycol based) (22g) Impetigo: Apply to affected area q 8 hours for 3 to 5 days. The SNP of 5% PVP-I was also significantly more effective than mupirocin against low-level and high-level mupirocin-resistant MRSA isolates in this porcine model ( P < 0. 2, 5. Dec 14, 2023 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Mupirocin ointment is not for intranasal, ophthalmic, or other mucosal use [see Warnings and Precautions (5. DrugBank Accession Number. Apr 11, 2022 · Therefore, an alternative mechanism to mitigate the further emergence of resistance could be to combine mupirocin with another antibiotic, thereby enhancing the antibacterial effect through synergistic activity, while enhancing the wound healing activity through the increased production of growth factors in keratinocytes by mupirocin. Sodium and chloride — major electrolytes of the fluid compartment outside of cells (i. Clotrimazole. 05), with significant bactericidal effects evident only after 12 h (Fig. Puromycin is a naturally occurring aminonucleoside antibiotic that inhibits protein synthesis by ribosome-catalyzed incorporation into the C-terminus of elongating nascent chains, blocking further extension and resulting in premature termination of translation. 85 episodes per 1000 catheter-days, respectively; NS). 0. Mechanism of action Mupirocin is a novel antibiotic produced through fermentation by Pseudomonas fluorescens. Nov 8, 2023 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Cefiderocol is a novel siderophore cephalosporin that exhibits remarkable antibacterial activity. 7, s From Feb 17, 2024 · Cephalosporins are β-lactam antimicrobials used to manage various infections caused by both gram-positive and gram-negative bacteria. Action. Sep 7, 2022 · Mupirocin Mechanism of Action. , extracellular) — work together to control extracellular volume and blood pressure. 05 Mechanism of action. When applied to intact skin, fusidic acid penetrates more rapidly than other antibiotics. 6)]. ACTION AND CLINICAL PHARMACOLOGY Mechanism of Action Mupirocin is a novel antibiotic produced through fermentation of Pseudomonas fluorescens. Feb 1, 2023 · Mechanism of Action. aureus and S. 3%. The base can be toxic Dec 5, 2018 · The mupirocin biosynthesis genes and the proposed pathway of mupirocin production. Microbiology: Mupirocin is an antibacterial agent produced by fermentation using the 28 organism Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. e. Antibacterial. Mechanism of action Acenocoumarol inhibits vitamin K reductase, resulting in depletion of the reduced form of vitamin K (vitamin KH2). Applied as an ointment to the skin or mucosa. The biosynthetic pathway belongs to the trans-AT group in which acyltransferase activity is provided by a separate polypeptide rather than in-cis as found in the original type I polyketide synthases Nov 25, 2021 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Mupirocin differs from other antibiotics in its synthesis, structure, and mechanism of action. Linezolid Mechanism of action. Apr 29, 2020 · Mechanism of Action. Half-life: 20-40 min. Curtis NA, Orr D, Ross GW, Boulton MG. Oil-in-water emulsion was prepared using Tween-20 and Span-20 (3–4% w / w) as emulsifying agents and liquid paraffin (5% w / w) as an oily phase with stirring at 1,800–2,000 rpm for 20 min. Background. Google Scholar; 2. Mupirocin is highly protein bound (greater than 97%) and the effect of wound secretions on the minimum Sep 27, 2023 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. The alarmone (p)ppGpp induces the stringent Mar 12, 2024 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Disease state based dosing: Renal failure: Dosing adjustments not necessary Dec 20, 2021 · Mupirocin was administered intravenously the first time with a distinctive mechanism of action that resulted in a better approach for the treatment of resistant bacterial infection. A screen of a Food and Drug Administration (FDA)-approved drug library revealed that the antibiotic neomycin sulfate potentiates the antimicrobial activity of mupirocin, whereas other library antibiotics did not. It is caused by specific mutations in gyrase and topoisomerase IV that weaken interactions between quinolones and these enzymes. Jan 11, 2024 · Mechanism of Action. Mar 3, 2014 · Target-mediated resistance is the most common and clinically significant form of resistance. The drug is a unique antimicrobial agent because of its structure and mechanism of action. Mupirocin is bactericidal at concentrations achieved by topical administration. Mometasone furoate diffuses across cell membranes to activate pathways responsible for reducing inflammation Label 1,2,3,14,15. 6. 07 0. 14 0. Rapidly inactivated after absorption, systemic levels are undetectable. Mechanism of Action. Inhibition of bacterial protein synthesis through interference with amino acid transfer from aminoacyl-sRNA to protein on the ribosomes. aureus to mupirocin, the drug of choice for nasal decolonization. 2014). The base can be toxic Jan 31, 2022 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. 06 at 6 h; P 0. As a small molecule, iodine It inhibits bacterial protein and RNA synthesis by reversibly binding to bacterial isoleucyl-tRNA synthetase. Aug 20, 2020 · Conversely, mupirocin had a slower onset of action (5. 06 at 6 h; P > 0. The 5 generations of cephalosporins demonstrate efficacy in treating skin and soft tissue infections, pneumonia, meningitis, and other infections. Mupirocin inhibits bacterial protein synthesis by reversibly and specifically binding to bacterial isoleucyl transfer RNA (tRNA) synthetase. g. aureus based on minimum inhibitory concentrations (MICs). 05), with significant bactericidal effects evident only after 12 h . aureus. neomycin C. Affinities of penicillins and cephalosporins for the penicillin‐binding proteins of Escherichia coli K‐12 and their antibacterial activity. 2A)(47). Povidone Iodine: Properties, Mechanisms of Action, and Role more, mupirocin-based nasal decolonization decreases infections among patients in high-risk settings, including surgery, intensive Mar 1, 2010 · Here, Thomas and colleagues describe the mechanisms underlying the mode of action and biosynthesis of mupirocin and discuss how this understanding could lead to the development of novel antibiotics. Mupirocin formulations are commercially available drugs used to treat skin wound infections in operative wounds, burn wounds, and diabetic wounds. It is used to treat or prevent infections caused by S. with permission from the publisher) [9]. Feb 20, 2011 · Mupirocin is a polyketide antibiotic produced by Pseudomonas fluorescens. Generic Name. Adverse effects. Fusidic acid works by interfering with bacterial protein synthesis, specifically by preventing the translocation of the elongation factor G (EF-G) from the ribosome. Clotrimazole is a topical broad-spectrum antifungal agent used for the treatment of a wide variety of dermatophyte infections and candidiasis. The chemistry, mechanism of action, antimicrobial activity, pharmacokinetics, clinical efficacy, adverse effects, dosage, and administration of mupirocin are reviewed. Mupirocin is used topically to treat skin infections caused by streptococci or staphylococci (e. The pharmacokinetics of mupirocin has not been studied in 26 individuals with renal insufficiency. May 1, 1989 · Both treatments were similarly effective with 97% patients treated with mupirocin and 93% patients treated with sodium fusidate responding. Jan 11, 2024 · Mupirocin belongs to the topical antibiotic class of medications and is utilized for managing and treating various skin and soft tissue infections. Cocaine produces anesthesia by inhibiting excitation of nerve endings or by blocking conduction in peripheral nerves. Pharmacology: Pharmacodynamics: Mechanism of action: Mupirocin is a novel antibiotic produced through fermentation by Pseudomonas fluorescens. , impetigo, nasal carriers of methicillin-resistant S. Bactericidal; inhibits protein synthesis in susceptible bacteria by reversibly binding to bacterial isoleucine-tRNA ligase (isoleucyl-tRNA synthetase), the enzyme that catalyzes the formation of isoleucyl-tRNA from isoleucine and tRNA. These results suggest that mupirocin could have clinical utility for superficial infections caused by Nov 27, 2023 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. pyogenes, and to eradicate nasal colonization with MRSA. Pharmacodynamics Mupirocin is bacteriostatic at low concentration but becomes bactericidal at concentrations achieved locally by topical administration. Side effects are uncommon and mild with mupirocin. Mupirocin is believed to exert its antimicrobial activity by inhibiting isoleucyl-transfer RNA, thereby obstructing bacterial protein and RNA synthesis, ultimately resulting in cell death. The SNP of 5% PVP-I was also significantly more effective than mupirocin against low-level and high-level mupirocin-resistant MRSA isolates in this porcine model ( P Oct 27, 2022 · Mupirocin is an antibiotic ointment that is used topically to treat infections of impetigo, MRSA, and staph infections. Metabolism The mechanism of action of mupirocin differs from other clinical antibiotics, rendering cross-resistance to other antibiotics unlikely. Apr 24, 2020 · Puromycin structure and mechanism of action. Mupirocin. 5. Mupirocin is available as a cream and an ointment. Absorption Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. In vitro, mupirocin possesses antimicrobial activity against staphylococci, streptococci, Hemophilus influenzae, and Neisseria Apr 1, 1999 · It also inhibited the isoleucyl-tRNA synthetase from Candida albicans, indicating a mechanism of action similar to that in bacteria. 1 ) and mechanism of action. Staphylococcus aureus is the second most frequent cause of nosocomial bloodstream infections worldwide []. It has bacteriostatic effects at minimum inhibitory concentrations and bactericidal effects at higher Nov 24, 2023 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Linezolid interferes with protein synthesis by binding to a unique RNA site on the 50 S subunit, preventing formation of the functional 70 S complex. ATC code: D06AX09. Mechanism of action Jan 17, 2023 · Mupirocin resistance can also be attributed to the development of bacterial genes that provide micro-organisms protection from mupirocin’s mechanism of action, especially in S. The nasal spray may be prescribed for individuals that come into contact with patients infected with MRSA, or other infectious diseases. Apr 10, 2022 · Therefore, an alternative mechanism to mitigate the further emergence of resistance could be to combine mupirocin with another antibiotic, thereby enhancing the antibacterial effect through synergistic activity, while enhancing the wound healing activity through the increased production of growth factors in keratinocytes by mupirocin. Aug 1, 2021 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Jan 1, 2019 · Mupirocin (MUP), bactroban, or pseudomonic acid is a natural crotonic acid derivative drug extracted from Pseudomonas fluorescens which is produced by modular polyketide synthases. Alevazol, Dermacinrx Therazole Pak, Lotriderm, Lotrimin, Lotrimin AF, Lotrisone, Mycelex. Further, the results stipulated that nano-mupirocin extended the topical application of mupirocin to the systemic application for the treatment of MRSA infections Mechanism of Action: Mupirocin, an antibiotic produced by fermentation of Pseudomonas fluorescens, inhibits bacterial protein synthesis by reversibly and specifically binding to bacterial isoleucyl transfer-ribonucleic acid (tRNA) synthetase. Pharmacology:Pharmacodynamics: Mupirocin is an antibacterial that inhibits bacterial protein synthesis by binding to isoleucyl transfer RNA synthetase. aureus). Mupirocin is supplied as a 2% solution in a water-soluble ointment base of polyethylene glycol. It has a broad antibacterial activity on Gram positive and some Gram negative bacteria, and a limited systemic adsorption: the half-life is 15 min, being converted into monic acid Dec 1, 2023 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. F. What will the nurse Mechanism of Action. Mupirocin inhibits isoleucyl transfer-RNA synthetase, thereby arresting bacterial protein synthesis. aureus (MRSA) is a particular problem in these high risk patients due to the limited therapeutic drugs Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Conversely, mupirocin had a slower onset of action (5. In Therapeutic Effects. 05), with significant bactericidal effects evident only after 12 h (Fig. A. Mupirocin is recognized as the most widely used topical antibiotic globally for treating Apr 24, 2020 · Abstract. Resistance occurs rarely. Mechanism of Action . It is associated with a low incidence of It is also active against certain gram-negative bacteria. Pharmacology: Pharmacodynamics: Mechanism of Action: Mupirocin is a novel antibiotic produced through fermentation of Pseudomonas fluorescens. Susceptible bacteria are Staphylococcus aureus [including methicillin-resistant (MRSA) and beta-lactamase-producing strains], Staphylococcus epidermidis, Staphylococcus saprophyticus, and Streptococcus pyogenes. Due to this particular mode of action, and its unique chemical structure, mupirocin does not show any Dec 11, 2023 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Protein bound: 95-97% in vitro. Due to this particular mode of action, and its unique chemical structure, mupirocin does not show any Cocaine is a local anesthetic indicated for the introduction of local (topical) anesthesia of accessible mucous membranes of the oral, laryngeal and nasal cavities. This drug has Feb 21, 2020 · Therefore, expression profiling is invaluable to the antibiotic discovery process as it simultaneously allows for dereplication and provides a detailed profile of the mechanism of action. Mupirocin was originally developed in the early 1970s and is derived from Pseudomonas fluorescens. Metabolism: liver; CYP450; <0. It possesses a similar mechanism of action to aminocaproic acid but is approximately 10-fold more potent. Plasmid-mediated resistance results from extrachromosomal elements that encode proteins that disrupt quinolone–enzyme Three antibiotics that have been used topically in humans and animals are fusidic acid, mupirocin and bacitracin, all of which have a narrow antibacterial spectrum. No exit-site infections occurred. Due to this unique mode of action, mupirocin demonstrates no in vitro cross-resistance with other classes of antimicrobial agents. 2. It is mainly bacteriostatic at low concentrations, although it is usually bactericidal in the high concentrations achieved by the topical application to the skin. Mupirocin inhibits bacterial protein synthesis by binding to bacterial isoleucyl transfer RNA synthetase. During translation elongation, aa-tRNA enters the A-site and accepts Action. 14 ± 0. 27 . Preliminary mechanism of action studies indicate that Mechanism of action. Apr 10, 2018 · Generic Name Triamcinolone DrugBank Accession Number DB00620 Background. 3 DOSAGE FORMS AND STRENGTHS Each gram of mupirocin ointment USP contains 20 mg mupirocin in a water Mar 20, 2020 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. Tranexamic acid is a synthetic derivative of lysine used as an antifibrinolytic in the treatment and prevention of major bleeding. Beauduy CE, Winston LG (2024): Miscellaneous Antimicrobial Agents; Disinfectants, Antiseptics & Sterilants (Chapter 50). Triamcinolone is a corticosteroid used to treat various inflammatory conditions in the body from allergic rhinitis to acute exacerbations of multiple sclerosis. The categories of mupirocin susceptibility have been described for S. Jan 7, 2008 · The incidences of catheter-associated bacteremia in honey-treated (n = 51) and mupirocin-treated (n = 50) patients were comparable (0. Brand Names. It is active against penicillinase-producing and methicillin-resistant strains of S. Mar 12, 2024 · Mupirocin is an RNA synthetase inhibitor antibacterial produced by fermentation using the organism Pseudomonas fluorescens. It was first patented in 1957 2 and received its initial US approval in 1986. It is most commonly known as a selection marker for cell lines genetically 24 intravenous administration of mupirocin was 20 to 40 m inutes for mupirocin and 30 to 25 80 m inutes for monic acid. During the study period, 2% of staphylococcal isolates within the hospital were mupirocin resistant. S. Mupirocin specifically and reversibly binds to bacterial isoleucyl transfer-RNA (tRNA) synthetase, which is an enzyme that promotes the conversion of isoleucine and tRNA to isoleucyl-tRNA. Mechanism of action, resistance, properties Pseudomonic acid A represents 90–95% from the mixture and is the main compound of Mupirocin (Bojarska et al. Mechanism of Action: bacteriostatic or bactericidal, depending on susceptibility and concentration; reversibly binds to isoleucyl-tRNA synthetase, inhibiting protein synthesis (monoxycarbolic acid) Mar 14, 2020 · Introduction. 97 versus 0. Because of its unique mechanism of action, there is no cross-resistance between mupirocin and other antimicrobial agents. (B) Basic mechanism of puromycin action. 11 It is one of the most common skin and mucous membrane antiseptic agents in use today. Mupirocin apparently exerts its antimicrobial activity by reversibly inhibiting isoleucyl-transfer RNA, thereby inhibiting bacterial protein and RNA synthesis. The mechanism of action is protein synthesis inhibition by binding to isoleucyl-tRNA synthetase. uv em hh ct ly yr oz vy ox wd